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KMID : 0364820090450040425
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Korean Journal of Microbiology
2009 Volume.45 No. 4 p.425 ~ p.429
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Detection of plcR-papR Genes by PCR in Identifying Enterotoxin Genes-Harboring Bacillus cereus Strains
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Yun Suk-Hyun
Kim Yong-Sang
So Soon-Ku
Jeong Do-Youn
Hahn Kum-Su
Uhm Tai-Boong
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Abstract
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Identification of virulent Bacillus cereus strains was examined by PCR using primers specific for the detection of plcR-papR, which encode regulatory proteins controlling the transcription of virulence factors in B. cereus. Total 96 strains of B. cereus that carried at least one of diarrheal toxin genes including hblACD, nheABC, and cytK showed all positive PCR products, while other 48 Bacillus strains that lacked the toxin genes were plcRpapR- negative. This PCR method targeting the plcR-papR genes appears to be simple and effective in identifying the enterotoxin genes-harboring B. cereus strains.
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KEYWORD
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B. cereus, enterotoxin genes, papR, PCR primer, plcR
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